SHITTU KOLAWOLE HAMMED

Microbiology

At the Microbiology department office

Appointment on Visitation important

Topic: Incidence, Antibiotic Susceptibility Pattern And Plasmid Profile Of Bacterial Pathogens From Surgical Site Infections In A Tertiary Hospital In Lagos, Nigeria

Description:

Surgical site infections [SSIs] remain a common postoperative complication despite the use

of prophylactic antibiotics and other preventive measures, mainly due to increasing antimicrobial

resistance. SSIs increase postoperative morbidity and mortality and may require hospital

admission, intravenous antibiotics and even surgical re-intervention. A hospital based

descriptive study was conducted on 100 consented postoperative patients with clinical SSIs.

Data on patients was obtained using structured data collection form. Swab samples were collected

aseptically from each patients. Bacteriological culture examination and identification was

done following standard microbiological techniques. Antibiotics sensitivity test was done by

Kirby-Bauer disc diffusion method. Ninety (90%) bacterial isolates were recovered from surgical

site infection. Gram negative bacteria (GNB) were predominant (83.3%) with the dominant

being Escherichia coli (27.78%) and Staphylococus aureus (16.67%). All the isolates were highly

resistant to amoxicillin/clavunalate, ceftazidime, cefuroxime, levofloxacin and all the isolates

were resistant to metronidazole but susceptible to imipenem, polymycin B and amikacin. The

plasmid analysis in this study revealed that out of the 40 (44.4%) multi-drug resistance isolates,

35 (87.5%) of which were Gram-negative bacteria had 9 (22.5%) detectable plasmid pattern

with the molecular weight of between 2027kbp to 23120kbp while the remaining 26 (74.6%)

had no plasmid bands. The remaining 5 (12.3%) which was Staphylococcus aureus isolates had

2 (40%) detectable plasmid pattern with the molecular weight of between 23130kbp and 6557

while the remaining 3 (60%) had no plasmid bands. Imipenem is the drug of choice in the treatment of surgical site infections in this study area These findings necessitates judicious antibiotic use and calls for surveillance of

SSIs periodically as well as strict adherence to good sanitation practice to reduce spread of drug-resistant pathogens.

#	Certificate	School	Year
1.	M.Sc (Medical Microbiology)	University of Lagos, Akoka, Lagos State, Nigeria.	2015

Serological and Molecular detection of West Nile virus in Selected Avian Species (Chickens, Ducks and Pigeons) in Lagos State, Nigeria.

Introduction

Arboviruses represent an increasing threat to global public health due to their ability to establish and spread rapidly once introduced into new areas with abundant competent vectors and susceptible hosts (Girard et al., 2020). Members of the flavivirus genus are transmitted by vectors, notably by mosquitos and ticks. The genus is comprised of several important human and animal viruses like the yellow fever virus (YFV), dengue virus (DENV), Japanese encephalitis virus (JEV), Saint Louis encephalitis virus (SLEV), and West Nile virus (WNV).

Aim of the Study: To detect the West Nile Virus in selected avian species (ducks, pigeons and chickens) using serological and molecular techniques.

Objectives of the Study

1. To determine the seroprevalence of the West Nile Virus antibodies in ducks, pigeons and chickens using ELISA

2. To detect WNV RNA in samples collected from avian species using reverse transcription polymerase chain reaction (RT-PCR);

3. To compare the prevalence of WNV across the three bird species

4. To generate baseline data that can guide surveillance, control and public health interventions.

SHITTU HAMMED is a Assistant Lecturer at the Department of Microbiology

SHITTU has a M.Sc in Medical Microbiology from University of Lagos, Akoka, Lagos State, Nigeria.