Associate Professor / Reader
At the Chemistry department office
Appointment on Visitation important
Topic: Extraction And Characterisation Of Collagen From Croaker Fish Scales
Proteins (including collagen) or peptides are very important bioactive compounds that can be used in food and medicine. Collagen has been extracted from bovine and porcine skin and bones. However, collagens obtained from porcine skin or bone are prohibited for some religious groups. Therefore, the utilization of fish processing waste through bioconversion into high-grade products like bioactive peptides would be a better alternative.
Aim & Objective
To extract Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) from croaker collagen method.
To identify the types of collagen present in croaker fish wastes.
To determine some physical properties of the extracted collagen and characterise the collagen using techniques.
Croaker scales were collected fresh from popular markets from Lagos. These scales were immediately washed with tap water and frozen until further used. The ASC was obtained after removing the non-collagenous protein using sodium hydroxide and demineralising with Na2EDTA. The resulted sample was treated with acetic acid, the mixture was filtered. The filtrate was subjected to salting and the precipitate was collected by centrifugation, the pellet was dialysed and the Collagen was freeze-dried by using a freeze-dryer.
The remaining matter from ASC extraction was subjected to extraction to obtain PSC using acetic acid in the presence of porcine pepsin. Thereafter, the mixture was filtrated, precipitated, dialysed and freeze-dried as previously described for ASC. All processes were done at 4 oC.
The extracted ASC and PSC were subjected to various characterisations. Amino acid composition was analysed. FTIR spectra of both collagens were obtained using total
reflectance-Fourier transform infrared (ATR FTIR) spectroscopic analysis. Differential scanning calorimetry (DSC) was used to determine the maximum transition temperature (Tmax) and total denaturation enthalpy (H) of the collagen.
Contribution to knowledge
Generation of alternative sources of collagen, especially from aquatic animals such as fish scales.
|1.||Ph.D (Analytical/Environmental Chemistry)||University of Ibadan, Ibadan, Nigeria||2014|
Adsorption of divalent metals (Cd (II) , Cu(II) , Ni(II) and Pb(II) ions from aqueous solution by agricultural waste materials
Water is of utmost importance in our daily lives, hence the need to improve and preserve its quality is growing continuously. Agricultural waste materials (AWMs) are widely used for the purification of wastewater and vary greatly in their ability to remove metals from solution. These AWMs are available in abundance as agricultural waste/agri-food by-products, hence the need for the beneficiation/valorization of such waste materials.
Use of AWMs such as beans husk (BH), plantain peel (PP), groundnut husk (GH) and fish scale (FS) for removal of four divalents (Cd (II) , Cu (II), Ni (II) and Pb(II) ) ions from aqueous solution.
Characterisation of the AWMs using techniques such as Fourier transform infrared (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and X-ray diffraction (XRD) shall be performed. The optimisation of the following variables: pH, adsorbent dosage, initial metal concentration, contact time and temperature to be investigated. The experimental data to be applied to the Langmuir, the Freundlich and the Temkin sorption isotherms. The kinetic study and the thermodynamic equilibrium model will be used to estimated.
The various biopolymers materials were sorted, dried and pulverised and sieved with 2mm mesh size stainless steel sieve. The characterisation of the AWMs shall be performed using the techniques stated at the objectives section. The protocols of Onwordi et al, 2017.
The functional groups present in the various AWMs were identified. The major functional groups present in the AWMs are hydroxyl, amide I, amide II and carboxylic acid. The morphology of the AWMs will be deduced with the aid of SEM. The thermal properties of the AWMs will be determined by use of TGA technique.
The optimum pH, adsorbent dosage, initial metal concentration, contact time and temperature will be obtained for the divalent ions using the AWMs.
ONWORDI CHIONYEDUA is a Associate Professor / Reader at the Department of Chemistry
ONWORDI has a Ph.D in Analytical/Environmental Chemistry from University of Ibadan, Ibadan, Nigeria