OGHENE OKIKIADE

Physiology

At the Physiology department office

Appointment on Visitation important

#	Certificate	School	Year
1.	Ph.D (Physiology)	University of Lagos	2024

The effect of acute restraint stress on oxidative stress markers in perimenopausal rat models after L-Arginine supplementation

 Introduction

Perimenopause was induced in female Sprague-Dawley rats using low dose 4 -Vinylcyclohexene Diepoxide (VCD), which resulted in the depletion of ovarian follicles, irregular estrous cycles, and hormonal imbalances.

Methodology

Sixty-three (63) female Sprague-Dawley rats were divided into three groups (n = 21): Control (28 days old), injected with Corn oil for 15 days; VCD (28 days old), injected with VCD (160 mg/kg BW) diluted in Corn oil for 15 days; and Aging rats (120 days old). Each group was subdivided into unstressed, stressed, and stressed groups that were supplemented with oral L-Arginine (100 mg/kg BW) for 60 days (n = 7). After exposure to acute restraint stress, blood samples were obtained via cardiac puncture and centrifuged. The serum was preserved for hormonal assay and biochemical assessment.

Results and Conclusion

L-Arginine supplementation significantly reversed the reduction in serum nitrate and nitrite levels caused by acute restraint stress in perimenopausal rats, suggesting its potential to regulate stress responses.

These findings suggest that L-Arginine supplementation may modulate stress responses in perimenopausal rats, particularly in VCD perimenopausal rats.

Contribution to Knowledge

L-Arginine supplementation can boost nitric oxide levels even under restraint stress condition, highlighting its ability to reverse nitric oxide levels caused by sudden restraint stress

OGHENE OKIKIADE is a Lecturer II at the Department of Physiology

OGHENE has a Ph.D in Physiology from University of Lagos