OBAYORI OLUWAFEMI SUNDAY

Microbiology

At the Microbiology department office

Appointment on Visitation important

Topic: Metagenomics Of Contaminated Environmental Compartments

Description: Physicochemistry, microbial community structure and functional characterization of animal charcoal polluted sites and underground waters. Metagenomic insight into the spread of antibiotic resistance genes amongst clinically important, poultry farms and environmental bacteria in Lagos State. Heavy metals and antibiotics resistomes of pristine and polluted environments. Cooccurence and coselection of hydrocarbon degradation, heavy metal resistance and antibiotic resistance genes in soil and aquatic ecosystems.

#	Certificate	School	Year
1.	Ph.D (Environmental Microbiology)	Microbiology, University of Lagos	2008

Metagenomic Approach to Monitoring Changes in Community Structure During Biostimulation of Hydrocarbon Polluted Sites with Agrowastes

Pollution of the environment with petroleum hydrocarbons is of major concern to individuals and governments because of the deleterious effects of the pollutants on human and environmental health. Indiscriminate use and disposal of petroleum hydrocarbon cuts is common, particularly in third world countries like Nigeria. Bioremediation is increasingly becoming a more acceptable cleanup approach because of its advantages over chemical and physical methods. Biostimulation with inorganic nutrients is a useful strategy in this regard because hydrocarbons are poor sources of mineral nutrients and such nutrients are a rate limiting factor in their biodegradation. Nutrient addition by way of use of organic and agro-wastes is considered a cheap and readily applicable strategy. Several agrowastes, including banana peels, plantain peels, animal manure and so on have been demonstrated to be good materials for biostimulation. Such approach requires effective monitoring and establishment of baseline parameters that could be useful in optimization, particularly monitoring of residual hydrocarbon, physicochemical changes and changes in community structure. Methodology Pretest-Posttest experimental design microcosm with control will be used. The soil physicochemistry will be evaluated using standard analytical protocols. Residual hydrocarbons in soil microcosms will be determined using gas chromatography. Samples for DNA extraction will be taken at the same interval. Changes in community structures in experimental and control soils will be monitored by metagenomic taxonomic and functional analysis. Combination of GRAPHPAD, STAMP and other metagenomic specific and non-specific tools will be used to analyze the data obtained.

OBAYORI OLUWAFEMI is a Professor at the Department of Microbiology

OBAYORI has a Ph.D in Environmental Microbiology from Microbiology, University of Lagos