MORO DAUPHIN DIGHITOGHI

Microbiology

At the Microbiology department office

Appointment on Visitation important

Topic: Epidemiology Of Salmonella And Other Enteric Bacterial Pathogens.

Description: I have carried out studies on isolation, identification and characterization of bacterial pathogens with emphasis on Salmonella and other enteric bacterial pathogens using standard microbiological techniques. Selective media such as Salmonella-Shigella agar, MacConkey agar etc. which were inoculated with stool samples collected aseptically into sterile universal bottles . These were taken to the laboratory within one hour for inoculation into the media and incubated at 37 degree centigrade for 18 to 24 hours. The isolates were identified with cultural, morphological and biochemical characteristics using reference manuals such as Bergeys manual of determinative bacteriology (1974) and Cowan and Steels manual for the identification of medical bacterial(1985). Antibiotic susceptibility of the bacterial was carried out using the disc diffusion technique. Serological study on the Salmonella isolates was carried out to further authenticate and confirm the different Salmonella serovars. In addition, plasmid profile of the Salmonellae was carried out using the method of Zhou et al (1990) with standard marker and gel electrophoresis. The Salmonellae were further identified using polymerase chain reaction with thermocycler, oligonucleotides and Taq polymerase. This method involved denaturation, annealing and amplification of the identified DNA. Similarly, studies were carried out on lower genital infections in infertile band pregnant women in Ile - Ife, bacterial pathogens and parasites among vendors in Ado Ekiti and Ajegunle , antibacterial effect of chewing sticks on oral bacteria among others. Seroprevalence of HIV was carried out on women in Oshogbo and Lagos respectively. Studies on molecular epidemiology of enterococci and enteric pathogens such as Shigella species, Proteus species and Enterobacter species are in progress. Attempt would be made to identify specific genes in these bacteria that are responsible for their very high multiple antimicrobial resistance which has created a lot of treatment challenges to Physicians, thus constitute a health hazard of global significance. Further attempts would be made to investigate production of possible vaccine against such enteric pathogens. Production of primers that are specific to such local enteric bacteria shall also be investigated. Antimicrobial resistance pattern of fungi associated with urinary tracts infections are also being studied with an attempt to discover drugs of choice for effective treatment and management of such infections among our population. Studies on dermatophites among pupils is also being carried out in order to evaluate the isolation and characterization of fungi implicated. The antimicrobial susceptibility pattern against most common used anti-fungal drugs would also be assessed to determine the most effective drugs for the treatment and control of such dermatophites. Studies using laboratory mice would also be carried out to determine the pathogenicity and virulence of such pathogenic fungi.Pulse Field Gel Electrophoresis (PFGE) of the Genomic DNA of Salmonella typhi shall be carried out on Salmonella typhi isolates from patients in Lagos Metropolis. Restriction Endonucleases Analysis Pattern generated by PFGE shall be analyzed and compared with Multidrug Salmonella typhi. Each restriction analysis pattern obtained by PFGE shall be confirmed to be stable and reproducible with repeated digestion of the DNA preparations. Further molecular studies shall be carried out on the S. typhi isolates using the qPCR analysis of multidrug S. typhi.

#	Certificate	School	Year
1.	Ph.D (Microbiology)	University of Ado-Ekiti, Ado-Ekiti	2003

Detection and molecular characterization and Trichophyton isolates among tinea capitis infected students and pupils in Lagos State

Scalp samples shall be collected aseptically from students and pupils having got ethical approval from relevant health authorities, Consent of both pupils and students would be sought and obtained from only those who fall within inclusion criteria while those who fall outside the criteria shall be prevented from taken part in the study. The subject shall as well be informed that the study is for research study and under strict confidentiality as none of their personal information should not be divulged to anyone. Fungi associated with tinea capitis shall be isolated using selective fungal media and identified by standard mycological methods or techniques. Molecular identification of such fungi shall be carried out using randomly amplified polymorphic DNA test (RAPD) and realtime-PCR for definitive identification of such fungi of medical significance. Anti-microbial susceptibility pattern of such fungi from students and pupils shall be carried out for effective treatment of tinea capitis.

MORO DAUPHIN is a Professor at the Department of Microbiology

MORO has a Ph.D in Microbiology from University of Ado-Ekiti, Ado-Ekiti