Meet LAWAL-SANNI Olajumoke Aminat, an Academic Staff of Lagos State University.
Microbiology Medical
Assistant Lecturer
Microbiology
At the Microbiology department office
Appointment on Visitation important
Topic: STUDIES ON MULTIPLE DRUG RESISTANT ON P. AERUGINOSA ISOLATED FROM DIFFERENT SOURCES IN LAGOS STATE, NIGERIA
Description: Introduction:Pseudomonas aeruginosa is one of the causes of hospital-acquired infections in the critically ill. It is an opportunistic pathogen that is the main cause of chronic lung infections in cystic fibrosis patients. Vulnerable people, like the immunocompromised individual, are particularly susceptible to infections.Aims:The aim of this study was to determine the prevalence, antibiotic resistance, and genetic profiles of P. aeruginosa from a variety of sources wound swap, inanimate surfaces, ventilators, water, urine, and blood sample in hospitals, water bodies from environment, and abbatoir water, animal waste and animal urine .And also to establish clonal relatedness and/or diversity utilizing advanced molecular techniques.Methods:In total, 3100 clinical samples, 900 environmental samples, and 500 animal sources will be used to detect the frequency of P. aeruginosa pathogens using cultural methods and identified by standard methods. Further identification will be carried out using AP1 20E. Antimicrobial susceptibility testing, ESBL and AmpC, and resistance determinants will be done using the standard recognized procedure. Virulence and resistance genes determinant will be done using specific gene markers. Possible Results:Positive culture will be reported of the samples using conventional culture, PCR, and Molecular methods. Multidrug-resistant P. aeruginosa will be found from different sources. The phylogenetic tree will be created and the clonal relatedness of P. aeruginosa from different sources will be determined. The results from this study will also provide information data on strains of P. aeruginosa circulating in Nigeria and will contribute to the global gene data bank thereby paving the way for a better understanding of resistance mechanisms and for instituting new intervention strategies in the areas of sanitation and waste management as well as novel antibiotics, diagnostic assay, and vaccine development.
| # | Certificate | School | Year |
|---|---|---|---|
| 1. | M.Sc (Medical Microbiology and Parasitology.) | University of Ilorin, Ilorin Kwara State. | 2021 |
PHENOTYPIC AND GENOTYPIC DETECTION OF MULTIPLE DRUG RESISTANT-PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT SOURCES IN LAGOS STATE, NIGERIA
Pseudomonas aeruginosa, a widely distributed bacterium in human, environment and animal populations has been listed among high priority2 pathogen by World Health Organization. The organism produces virulence factors such as pili, exotoxins, LasB and elastase, causing cystic fibrosis, urinary tract and wound infections in intensive care unit. Classes of antibiotic such as carbapenems, cephalosporin and flouroquinolones are used for treatment. The emerging multi-drug resistance MDR P. aeruginosa strains has contributed to treatment failure due to the production of extended-spectrum beta-lactamases ES L , ambler class C -lactamases AmpC , and carbapenemases CRPA , In Nigeria, P. aeruginosa has continued to be a public health concern. These health crises limit productivity and inflict economic consequences on impacted communities. This study determines virulotype and antimicrobial resistance determinants of MDR-P. aeruginosa from different sources. Hospital, animal and environmental samples will be cultured and genotypic identification of ESBL, AmpC, and CRPA genes in P. aeruginosa isolates will be done. Whole genome sequencing will be performed and bioinformatics tools will be used for clonal diversity and relatedness of the sequenced isolates. This study will enhance public health safety and well-being by educating the community and bolster the resilience of abattoir environments and hospitals through a One Health approach.
LAWAL-SANNI Aminat is a Assistant Lecturer at the Department of Microbiology
LAWAL-SANNI has a M.Sc in Medical Microbiology and Parasitology. from University of Ilorin, Ilorin Kwara State.
