JEWO PETER IMONI

Anatomy

At the Anatomy department office

Appointment on Visitation important

Topic: I. Reproductive Biology. Ii Surgical Pathology Of Burn Injuries In Male Fertility. Iii. Preservation Of Female Reproductive Potential

Description: i. Male reproductive toxicology: Either as lead investigator or as member of a team, my work in this area has concentrated on the effects of various substances including pharmaceutical agents such as atensunate and doxorubicin or physical conditions such as testicular varicoceles on the functional and structural integrity of the male gonad. This also includes investigation of various substances, especially of plant origin which act as anti- oxidants that can protect the testis from toxic damage.ii. Surgical Pathology of burn injuries in male fertility: I studied the consequences of severe burn injuries on spermatogenesis, androgenesis and the histological integrity of the testis in both animal models and human survivors of mass burn incidences. My team is, to our knowledge, the first to characterize in detail, the systemic effects of burn injuries on the histology of the testis, using animal models to overcome the challenge of getting testicular tissue from living humans. We also showed that ascorbic acid, when used as adjuvant therapy is useful in protecting the testis from this anti-fertility action. Consequently, Our article on the Protective role of ascorbic acid in burn-induced testicular toxicity in rats has been the Domain Definer since it appeared in the Journal, Burns in 2012, See BioMed Updater, WIPID-Who is Publishing In My Domain .

#	Certificate	School	Year
1.	Ph.D (Human Anatomy)	Department of Anatomy, University of Lagos	2012

Effects of different equilibration times on follicle viability in frozen-thawed mouse ovaries.

Introduction:Young women who need to undergo irradiation or take chemotherapy for the treatment of cancer will experience premature ovarian failure and menopause. The options for preserving fertility in these females include vitrification and freezing of ova, embryos or ovarian tissue. Vitrification of ovarian tissue offers several advantages over the others. This includes the high number of follicles, especially smaller follicles that can be recovered from this tissue. Preserving ovarian tissue however involves the application of several chemical substances in varying concentrations and duration of time. Various cryoprotectants CPs and equilibration regimens are used by practitioners. The optimum vitrification protocol that ensures maximum follicle recovery is yet to be determined.Aims/Objectives:i. To investigate the effects of altering equilibration times using well known CPs on follicle recovery in frozen-thawed mouse ovary Methodology: Ovaries from albino mice were used for this study. They were equilibrated in turns in 7.5% and 20% ethylene glycol EG , and Dimethyl sulfoxide DMSO , with 0.5M sucrose as dehydrant. My base medium was PBS supplemented with 10% BSA. They were then plunged into liquid nitrogen at -196 0C. Group A was fixed fresh and B and C were exposed to vitrification with 2 different durations of equilibration times.After three weeks, they were thawed in a water bath at 37 0C and prepared for histological examination. Results:Follicle recovery was comparable in both concentrations of CPs when compared with fresh ovaries. Preservation was better in the more primitive follicles compared to the more mature antral follicles.Contribution to Knowledge:This study shows that follicle recovery from vitrified ovaries is satisfactory in mice even at various equilibration times. Follicle may not be significant if minor alterations occur in duration of exposure inadvertently during preservation.

JEWO PETER is a Associate Professor / Reader at the Department of Anatomy

JEWO has a Ph.D in Human Anatomy from Department of Anatomy, University of Lagos