Meet FAJANA OMOLADE OLUSEGUN, an Academic Staff of Lagos State University.
Xenobiochemistry, Plant Biochemistry And Protein Chemistry
Lecturer I
Biochemistry
At the Biochemistry department office
Appointment on Visitation important
Topic: The Inhibitory Activity Of Cowpea Vigna Unguiculata L. Walp Protein Isolates On Some Diabetes Type 2 Related Enzyme
Description: Diabetes mellitus DM is a metabolic disease marked by a severe lack of insulin secretion as a consequence of islets of Langerhans atrophy Cowpea Vigna unguiculata L. Walp is a centuries-old human crop, having originated in Africa and spread throughout Latin America and Southeast Asia. Cowpea is known for its pharmacological activities, which include the treatment of diabetes, inflammation, and the like. This work is aimed at estimating the Vigna unguiculata seed protein isolate inhibitory activity on some type-2-diabetes-related enzymes like alpha amylase, alpha glucosidase, and lipase. The cowpea protein isolate was extracted using alkaline extraction and subsequent iso-electric precipitation. Protein determination was carried out by the Lowry method, and the enzyme inhibition assay and substrate concentration were determined using a standard method. The result indicates that the protein isolate has 22% recovery. The IC50s were 1.0, 1.40, and 1.20 for alpha amylase, alpha glucosidase, and lipase, respectively, which is very distinctively close to that of acarbose and orlistat. The inhibition was 76% at 1 mg/ml of Cpi for alpha amylase, 63% at 0.8 mg/ml of Cpi for alpha glucosidase, and 86% at 1 mg/ml of Cpi for lipase, while the mode of the inhibition of type-2-diabetes-related enzymes was noncompetitive, uncompetitive, and mixed competitive for alpha amylase, alpha glucosidase, and lipase, respectively. These results indicate cowpea protein isolate can effectively inhibit alpha amylase, alpha glucosidase and lipase inhibitors, which can be a good candidate in the treatment of type 2 diabetes can also be used in the designing of anti-diabetic drugs
| # | Certificate | School | Year |
|---|---|---|---|
| 1. | Ph.D (Biochemistry) | Biochemistry, Lagos State University | 2023 |
Determination of Heavy Metal Contaminants in Leafy Vegetables Cultivated by the Roadside
A study was conducted to analyse the heavy metals in some leafy vegetables cultivated by the roadside, including Talium triangulare, Amaranthus hybridus, and Telfairia occidentalis. The vegetables were sampled from different areas of Ojo local government. The vegetables were digested using 98% nitric acid HNO and analysed with the aid of an atomic absorption spectrophotometer AAS to determine heavy metals. The mean concentration for each heavy metal in the samples gotten from each site was calculated, and the comparison of these data was done amongst the three different locations. These were compared with the permissible levels set by the FAO and WHO. Results showed that the levels of copper and cadmium for the leafy vegetables ranged from 0.3944 0.159 to 1.6559 0.919 and from 0.0854 0.043 to 0.2563 0.249 mg/kg dry weight, respectively. While that of zinc and lead was 1.8028 0.111 to 6.2267 1.572 and 0.0856 0.040 to 2.104 1.490 mg/kg. When compared with standards, heavy metal levels were found to be within the safe limit, which is an indicator that the leafy vegetables in the Ojo local government area are safe for consumption without the risk of environmental toxicants.
FAJANA OLUSEGUN is a Lecturer I at the Department of Biochemistry
FAJANA has a Ph.D in Biochemistry from Biochemistry, Lagos State University
