Xenobiotic, Plant Biochemistry And Protein Chemistry
At the Biochemistry department office
Appointment on Visitation important
Topic: Biochemical Estimation Of Antioxidants Properties Of A Protein Isolate From Fermented Cucumeropsis Manni (melon) Seed On Streptozotocin-induced Diabetes Mellitus Rats.
Diabetes mellitus is a chronic metabolic disease characterized by hyperglycaemia due to insulin deficiency; or insulin resistance, or both. Hyperglycaemia, or raised blood sugar, occurs when the cells become unable to utilize glucose and/or the liver and skeletal muscles cannot store glycogen. World Health Organization (WHO) has recommended the evaluation of traditional plant treatments for diabetes as they are effective, non-toxic, with less or no side effects and are considered to be excellent aspirants for oral therapy. However, the use of protein isolate from a fermented legume, Cucumeropsis manni (melon) seed in the treatment of diabetes mellitus needs to be explored. This work seeks to find the use of the protein isolate in the treatment of diabetes mellitus and also possibly see if the protein isolate could improve physiologically parameters, such as elevated plasma lipid profiles, that are associated with metabolic diseases. Diabetes mellitus will be induced by intraperitoneal injection of 55mg/kg/ bodyweight of Streptozotocin dissolved in sterile normal saline into overnight fasted rats and treated thereafter with the protein isolate. The biochemical evaluation carried out will determine the effect of the therapeutic activities of the protein isolate. The result of lowering the blood glucose, lipids and associated genes in diabetic condition will then be a good indicator of the use of this nutraceutical for the management of diabetes mellitus. This will add value to the use of the legume in the management of diseases. It will also help as an alternative use of plants seed protein in the treatment of diabetes.
|1.||Ph.D (Biochemistry (In View))||Biochemistry, Lagos State University||2022|
In-vitro antioxidant activities of a protein isolate of Cucumeropsis manni (melon) seeds. (2, 2 diphenyl-1-picrylhydrazyl (DPPH), Nitric Oxide, Free radical scavenging activity
Diabetes is a disorder of carbohydrate, fat and protein metabolism attributed to diminished production of insulin or mounting resistance to its action. It is characterized by hyperglycaemia which occurs when the cells become unable to utilize glucose and/or when the liver and skeletal muscles cannot store glycogen. This work seeks to determine the antioxidant activity of protein isolate from Cucumeropsis manni (melon) seeds Protein was isolated from fermented melon seed-ogiri (Citrullus vulgaris) and purified by gel filtration. The results will indicate that the DPPH inhibitory activity of the protein, if the isolate increases with a proportional increase in the concentration of the protein, or there is a progressive increase in the nitric oxide inhibitory activity of the protein isolate of fermented Cucumeropsis manni (melon) seeds. Observation on the reducing activity of the fermented Cucumeropsis manni (melon) seeds protein isolate and the total antioxidant capacity. This will contribute to the management of diabetes owing to the antioxidant activity of the protein isolate.
FAJANA OLUSEGUN is a Lecturer II at the Department of Biochemistry
FAJANA has a Ph.D in Biochemistry (In View) from Biochemistry, Lagos State University