BANKOLE HABEEB ADEBODUN

Biochemistry

At the Biochemistry department office

Appointment on Visitation important

Topic: Molecular And In Silico Approach To Identify Putative Genes And Pathways Involve In Non-communicable Disease

Description: Introduction: Advancement in molecular biology and the advent of bioinformatics have provided means of identifying and understanding the complex relationship that exist among the most relevant physiological pathways of the onset and progression of most non-communicable diseases. Several genes involved in these diseases have been identified and stored in various databases. These databases can serve as means of curating candidate genes that could serve as biomarkers for screening or/and targets for therapeutics. Screening of biological compounds against these targets would help confirm folkloric claims of the efficacy of such compounds as well as could provide a means of identifying the inhibitory potentials of some active compounds against enzymes in disease conditions.Aim: to identify genes or their protein products that are directly or remotely linked with disease conditions that could be differentially expressed, and could serve as markers for diagnostic or prognosis purposes or targets for targets for therapeutic purposes.Methods: in silico identification of genes and pathways, expression and differential expression of genes will be done using PCR, protein expression analysis will be done using western blot and ELISA analysis, therapeutic potentials of natural products will be confirmed by checking their inhibitory potentials and understanding the mode of inhibition of target enzymes associated with disease conditions

#	Certificate	School	Year
1.	Ph.D (Biotechnology)	University of the Western Cape, Cape Town, South Africa	2016

DIPEPTIDYL PEPTIDASE-4 DPP4 INHIBITORY POTENTIALS OF THREE NIGERIAN SPICES

Introduction: Food and spices with dipeptidyl peptidase IV DPP-IV inhibitory property have been reportedly used in the treatment of diabetes mellitus and are regarded to be safer, cheaper, readily available, and does not have adverse effect in body mechanism compared to chemical based therapeutics. Compounds that inhibit the action of DPP-IV enzyme are novel drugs in the management of diabetes mellitus.Aim: This study aimed at evaluating the DPP-IV inhibitory potential of three Nigerian spices; Gongronema latifolium Utazi leaf , Monodora myristica Ehuru seed and Piper gineese Uziza Leaf, Seed, and Stem .Methodology: The spices were extracted with alcohol and double distilled water. The concentrated spices were reconstituted to different concentrations from the stock and in vitro analysis of the inhibitory effect of the extracts on DPP-IV was carried out using microplate reader. The phytoconstitents of the extract with the highest inhibitory effect was determined using HPLC-MS.Results: The IC50 of the standard Vidagliptin was the lowest 0.2849 g/ml and stastistically significant when compared to Monodora myristica which is the extract with the highest inhibitory effect. The inhibitory analysis of the stem, leaf and seed of Piper guineese that, the stem of P. guineese have a better inhibitory potential than the leaf and the stem. Also, all the ethanol extracts of the samples showed a better inhibitory activity than the aqueous extract except Monodora myristica whose aqueous extract showed better percentage inhibition than its ethanol extract. The phytochemicals analysis of Monodora myristica Ehuru seed revealed it contains various active compounds that need to be analyzed individually.Conclusion: There is a probable potential of these local spices possessing some active compounds that inhibits DPPIV

BANKOLE HABEEB is a Senior Lecturer at the Department of Biochemistry

BANKOLE has a Ph.D in Biotechnology from University of the Western Cape, Cape Town, South Africa