Clinical Anatomy And Oncology
At the Anatomy department office
Appointment on Visitation important
|1.||M. Phil. (CANCER SCIENCE)||GLOBAL HEALTH DEPARTMENT, STELLENBOSCH UNIVERSITY||2021|
Clomiphene Citrate Induced Histomorphometry and Histological Changes in the Ovary and Uterus of Adult Female Sprague-Dawley Rats
Background: Clomiphene citrate (CC) is used in the management of infertile female e.g., in PCOS. Histological and histomorphometry changes in the ovary and uterus is important in understanding the drop seen in ovulation/implantation ratio (80:13) from CC use.
Objective(s): To investigate the histomorphometry and histological changes in the ovary and uterus of adult female rats using different doses of CC.
Materials and Methods: Twenty-five female Sprague Dawley (SD) rats were divided into 5 groups. Group A received distilled water (10 ml/kg, p.o.), groups B -D were given 0.2, 2, 4g. Group E was given 6mg/kg/day of CC dissolved in distilled water. Administration was done orally at diestrus. Animals were sacrificed at the proestrus tor ovarian follicular diameter, uterine thickness and uterine histology.
Results: There was a significant increase in primary, secondary and tertiary ovaria diameter in all the treated rats (p<0.05) except for Group E. The effect of CC in Group D was increased compared to B and C(p>0.05). There was significant increase of uterine thickness in Group B compared to the control p<0.05). There was no significant lesion in the histology of the uterus. Group D and E showed reduction (p>0.05) in the uterine glandular
Conclusion: There is a dose-dependent increase in follicular function with Clomiphene, while there is a decrease of uterine activities except at very low dose. Therefore, low dose of CC favours fertility, while large doses and prolong use may be counterproductive.
BAMIRO AKEEM is a Lecturer II at the Department of Anatomy
BAMIRO has a M. Phil. in CANCER SCIENCE from GLOBAL HEALTH DEPARTMENT, STELLENBOSCH UNIVERSITY