AMISU OLUGBENGA KEHINDE

Microbiology

At the Microbiology department office

Appointment on Visitation important

Topic: Evaluation Of Rapid And Economical Diagnostic Methods For Detection Of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Bacterial Pathogens

Description:

Antimicrobial Resistance and exploration of phytochemiAntimicrobial resistance (AMR) remains a major public health challenge worldwide, particularly in developing countries where the burden of infectious diseases remains high, attributable tolow hygiene practices. The increasing prevalence of multidrug-resistant (MDR) bacterial pathogens such as extended-spectrum beta-lactamase (ESBL)-producing organisms has escalated identification and development of different alternative therapeutic agents. This research focuses on the antimicrobial potential of plant-derived bioactive compounds, especially essential oils, against clinically significant resistant bacteria.

The interest is aimed at (i) isolation and characterization of MDR bacterial pathogens from clinical and environmental sources, (ii) evaluate the antimicrobial efficacy of selected medicinal plant extracts and essential oils, and (iii) investigate their mechanisms of action, including membrane disruption and inhibition of virulence factors. Standard microbiological and molecular techniques, including culture-based assays, disk diffusion methods, minimum inhibitory concentration (MIC) determination, and polymerase chain reaction (PCR), will be employed.

It is hoped that the research outcomes will include the identification of potent plant-based antimicrobial agents and a better understanding of their interaction with resistant bacterial strains. This study will contribute to the development of alternative, cost-effective therapeutic options and support efforts to combat antimicrobial resistance. Ultimately, the findings will have significant implications for public health, drug development, and sustainable use of medicinal plants in Nigeria and beyond

cal Against Multidrug-Resistant Bacterial Pathogens

#	Certificate	School	Year
1.	Ph.D (Medical Microbiology)	University of Lagos UNILAG	2006

Antimicrobial activities of selected medicinal plants’ essential oils Wounds infection associated methicillin resistant Staphylococcus aureus

Staphylococci are normal bacterial inhabitants of the body surfaces with Staphylococcus aureus known to occasionally cause tissue damage infections by production of hydrolytic enzymes and many virulent factors. The World Health organization (WHO) now designated Staphylococcus as a global priority organisms due to their prevalence and wide spread plasmid encoded resistance to many therapeutic antibiotics, methicillin. Many medicinal plants are known for their richness in bioactive chemical compounds with varying characteristic antimicrobial activities, including Ocimum gratissimum , Xylopia aethiopica and Allium sativum. 

RESEARCH PROBLEMS

Wounds constitute risk factors for skin resident microbial flora, such as S. aureus to cause invasive localized or disseminated infections requiring effective antimicrobial intervention.

 AIM:

 To Determine Antibiotic sensitivity and Multidrug resistant strains by disk diffusion assay

 To screen isolates for methicillin resistance using oxacillin and cloxacillin antibiotics 

 To determine antimicrobial activity of selected test medicinal plant essential oils disk diffusion and double fold serial dilution techniques

METHOD

Would infections samples of statistically significant number will be collected from three general hospitals in the Lagos metropolis and cultured on selective and differential MRSA agar, disk diffusion method to determine their susceptibility to twelve commonly used antibiotics, methicillin resistance using oxacillin as indicator antibiotics. Molecular method will thereafter be used to detect and characterize the encoding genes on cell plasmids.

SIGNIFICANCE THE STUDY: The study is very important, especially in modern medicine where antibiotic resistance is increasing, and provide a cost-effective alternative to antibiotics, especially in Nigeria, where many medicinal plants are locally available.  

AMISU KEHINDE is a Senior Lecturer at the Department of Microbiology

AMISU has a Ph.D in Medical Microbiology from University of Lagos UNILAG