AJOSEH SAMUEL OLUWASEGUN

Microbiology

At the Microbiology department office

Appointment on Visitation important

Topic: Antimicrobial Resistance And Epidemiology Of Infectious Diseases

Description:

Brief Overview on Antimicrobial Resistance and Epidemiology of Infectious Diseases

The evaluation and quantification of the effects of bacterial, fungal, viral, and parasitic infections as well as the use of control, treatment, and prevention interventions to manage, avert, and ultimately eradicate infectious diseases seeks to improve health worldwide. To achieve this, advanced research tools such as Next Generation Sequencing or Whole Genomic Sequencing, Immunoinformatics, Bioinformatics among others are put into use for cuting edge research delivery.

#	Certificate	School	Year
1.	M.Sc (MICROBIOLOGY)	Lagos State University, OJO	2019

STUDIES ON MULTIPLE DRUG RESISTANT ACINETOBACTER BAUMANNI FROM FOOD ANIMALS, ENVIRONMENT AND HOSPITALIZED PATIENTS IN LAGOS, NIGERIA.

Introduction: Acinetobacter baumannii, a health-care-associated pathogen that causes outbreaks and is frequently encountered in ICU patients on mechanical ventilation, immunocompromised patients, post-surgical patients, and wounded/injured patient, requires immediate detection and treatment.

Aims: The present study aimed to detect the frequency of A. baumannii in clinical samples (wound swab, blood, sputum, nasal/aspirate sample and urine), environmental sample (lake water and landfill soil), and food animals’ sources (raw meat and cloacal/rectal samples) by Rapid Acinetobacter baumannii Detection- loop-mediated isothermal amplification (RABD-LAMP) assay in comparison with the different culture methods and clonal relatedness of the isolates from different sources.

Methods: In total, 2100 clinical samples, 900 environmental samples and 600 food animal sources will be used to detect the frequency of Acinetobacter baumannii, non-Acinetobacter baumannii and other members of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens using cultural methods, biochemical methods and molecular methods. Disc diffusion antimicrobial susceptibility testing and plasmid analysis will also be conducted using standard methods.

Results: Positive culture will be reported of the samples using conventional culture, PCR and RAPD-LAMP ASSAY methods. The most commonly identified pathogens will be reported from each of the samples of different sources. Multidrug-resistant A. baumannii will be found from different sources. The RABD-LAMP assay sensitivity and specificity will be determined when compared to conventional culture, and PCR. Phylogenetic tree will be created and clonal relatedness of Acinetobacter baumannii from different sources will be determined. The results from this study will also provide information data on strains of A. baumannii circulating in Nigeria and will contribute to the global gene data bank thereby paving the way for better understanding of resistance mechanisms and for instituting new intervention strategies in the areas of sanitation and waste management as well as novel antibiotics, diagnostic assay and vaccine development.

AJOSEH SAMUEL is a Assistant Lecturer at the Department of Microbiology

AJOSEH has a M.Sc in MICROBIOLOGY from Lagos State University, OJO