AJONUMA LOUIS CHUKWUEMEKA

Physiology

At the Physiology department office

Appointment on Visitation important

Topic: Reproductive Physiology & Endocrinology

Description: Interaction of reproductive tract epithelia and gametes, spermatogenesis & sperm maturation, ovulation, fertilization

#	Certificate	School	Year
1.	Ph.D (Reproductive Physiology/Physiology)	Department of Physiology, The Chinese University of Hong Kong	2004

The role of CFTR gene in cabergoline amelioration of ovarian hyperstimulation syndrome using a rat model.

Introduction: It has been demonstrated recently that suppression of CFTR by progesterone inhibits transepithelial transport of fluids during OHSS treatment with progesterone. Objectives: However, the mechanism of action by cabergoline has not been elicited. It is not known if carbagoline also suppress CFTR expression. Methods: Twenty female Sprague Dawley (SD) rats will be divided into 4 groups of 5 rats. Group A was the control no treatment, while groups B, C and D served as the test groups. Group A received 0.5ml of normal saline daily while groups B, C and D superovulated to induce OHSS. B receive also orally 0.5ml of normal saline daily while C and D progesterone 25mg/kg and D cabergoline for 4 days. Body weights obtained. Serum samples obtained from the rats will be assayed for reproductive hormones (Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Estradiol (E2)). Uterus for histology. We will use real-time reverse transcriptase chain reaction (RT-PCR) methods. Western blot analysis, immunofluorescence and light microscopy will also be used. Contribution to knowledge: The findings, will demonstrate CFTR involvement during OHSS treatment using cabergoline.

AJONUMA LOUIS is a Associate Professor / Reader at the Department of Physiology

AJONUMA has a Ph.D in Reproductive Physiology/Physiology from Department of Physiology, The Chinese University of Hong Kong