AJAYI GARIEL OLUKAYODE

Medical Biochemistry

At the Medical Biochemistry department office

Appointment on Visitation important

Topic: Phytomedicine, Drug Discovery, Biochemical And Environmental Toxicology

Description: My current research efforts are focused on biochemical, anti-oxidative and toxicological effects of medicinal plants and their uses for managing diseases such as diabetes, cancer, hypertension and nephrological problems. At the same time, I consider the identification, isolation and characterization of active components of the medicinal plants with the use of gas chromatography-mass spectrometry (GC-MS) and their biological activities in rats. I also conduct research in the area of Environmental Toxicology using medicinal plants as remedies for heavy metal poisoning.

#	Certificate	School	Year
1.	Ph.D (Biochemistry)	Department of Biochemistry, Federal University of Agriculture, Abeokuta	2014

Antioxidant activities and cytotoxic effects of Allium fistulosum L. extracts: Implications for anticancer potential

Cancer has become a global health burden and it is the most common non-communicable disease. Approximately 60% of drugs currently used for cancer treatment have been isolated from natural products and the plant kingdom has been the most significant source. Allium species like Allium cepa, Allium sativum, Zingiber officinale have been studied for the treatment of cancer, cardiovascular diseases, inflammation and oxidative damage. Hence, this study is being carried out to examine the antioxidant activities and cytotoxic effects of Allium fistulosum L. extracts and its implications for anticancer potential. Allium fistulosum is known as Welsh onion or spring onion and is used as nutritional condiment in fried rice preparation in Nigeria. Various solvents extracts (aqueous, hydroethanol, methanol, dichloromethane, ethylacetate) will be prepared. On the extracts, phytochemical screening, phenolic and flavonoid contents will be determined. The GC-MS analysis will be carried out. To evaluate the in-vitro antioxidant activities of the extracts, free radical scavenging and antioxidant activities of the extracts will be done by carrying out 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay, total antioxidant capacity (TAC), ferric reducing antioxidant (FRAP) assay,  B-carotene bleaching test, hydrogen peroxide scavenging ability test and scavenging ability test of nitric oxide. The effects of the extracts will be determined on Allium cepa root cell mitosis and the viability or proliferation of cell lines such as MCF-7 (breast cancer) and HT-29 (colon cancer) to investigate the genotoxicity and cytotoxity of the extracts. The antioxidant and cell viability parameters will be verified for the correlation with the phenolic and flavonoid contents of the extracts. It is being proposed that the antioxidant activities of the phytochemical compounds in A. fistulosum will induce the genotoxic and cytotoxic properties which may be a source of drug development against cancer.

AJAYI GARIEL is a Senior Lecturer at the Department of Medical Biochemistry

AJAYI has a Ph.D in Biochemistry from Department of Biochemistry, Federal University of Agriculture, Abeokuta