Nutritional Biochemistry, Neurobiochemistry, Anti-ageing Research.
At the Medical Biochemistry department office
Appointment on Visitation important
Topic: Animal Models In Phytopharmacology
The variety of species that the animal kingdom provides has remarkably helped in the advancement of scientific investigations, leading to land breaking discoveries that have made life better for the world's inhabitants. In the process of drug discovery, the use of animal models is an inevitability; they provide the needed platform that will help reveal, to a large extent, the physiological functions of phytochemical compounds. In this chapter, we review the various animal methods commonly used in evaluating candidate drugs from phytochemicals, the method of obtaining the models and the different disease conditions successfully modelled. Special attention is given to Drosophila melanogaster. We give an introduction to the biology of this model organism and an overview of its use to model human diseases and plagues. We describe the general process of using Drosophila in phytopharmacology studies and provide a compilation of resources that will help anyone wishing to utilize this organism.
|1.||Ph.D (Biochemistry)||Biochemistry Department, Kampala International University, Kampala, Uganda||2020|
Moringa oleifera not Ananas sativa Increases Learning and Memory as Age Progresses in Drosophila melanogaster
Ageing is associated with cellular senescence in a variety of organisms, it is an irreversible biological clock which is associated with the accumulation of reactive oxygen species (ROS) induced damage to macromolecules as postulated by the free radical theory of ageing, and the most vulnerable organ is the brain. Oxidative damage to the brain is due to high levels of polyunsaturated fatty acids in the cell membrane. Age-long accumulation of this oxidative damage leads to a decline in cognitive ability in a variety of organisms, however, the modulatory effect of Moringa oleifera and Ananas sativa on age-related memory decline is not well understood. Therefore, our aim was to examine the age-long effect of the oral supplementation of the plant extracts on age-induced memory decline and Paraquat induced oxidative stress. Moringa extract gave a modulatory effect on learning and memory with age, and also in its ability to rejuvenate the activities of antioxidant enzymes catalase and superoxide dismutase, with a significant decrease in lipid peroxidation products in the brain of aged flies 50 days old with respect to the young flies of 5 days old, there were no significant changes observed in the Ananas fed flies. Our result revealed the ameliorative role of Moringa oleifera bioactive components on olfactory conditioned learning and memory, oxidative stress resistance and suggests the potential anti-ageing impact of the extract on age-related memory decline in Drosophila melanogaster w1118 wild type flies.
AJAGUN-OGUNLEYE OLUFEMI is a Lecturer II at the Department of Medical Biochemistry
AJAGUN-OGUNLEYE has a Ph.D in Biochemistry from Biochemistry Department, Kampala International University, Kampala, Uganda