Reproductive And Endocrine Physiology
Lecturer II
Physiology
At the Physiology department office
Appointment on Visitation important
# | Certificate | School | Year |
---|---|---|---|
1. | Ph.D (Reproduction/Endrocrinology) | University of Lagos | NaN |
Effect of clomiphene citrate on sex hormones in female Sprague Dawley rats.
Abstract: Clomiphene citrate (CC) is used in the
management of infertile female. Hormonal imbalance contributes to this
problem. The aim of this study is to
evaluate how CC affects the distribution of sex hormones in rats.
Materials
and Methods: 25 female Sprague Dawley (SD) rats
were divided into 5 groups. Group A was the control. Groups B, C, D, E were the
treated groups. Group A received 0.5ml of sterile water at diestrus. Groups B,
C, D were given 0.2mg/kg, 2mg/kg, 4mg/kg all at diestrus. The last group (E)
was given 6mg/kg/day of cc dissolved in sterile water. Administration was done
orally. After six cycles of treatment, animals where sacrificed at different
phases, blood collected at the 4 phases of the estrus cycle for analysis.
Results:
This study shows that at a high dose, there
is a significant decrease in the serum estrogen and progesterone level compared
with control, No change with the lower doses versus control across all phases
except for estrus phase where progesterone increase. For FSH, LH, there is an
increase at high dose but a decrease at low dose except for proestrus and
estrus phases where the gonadotrophs increase.
Conclusion.
There is a dose dependent effect of clomid on sex hormones. The hormonal
pattern follows a negative feed back effect of the steroidal hormones on the gonadotrophins.
However, the increase of LH at proestrus and estrus phase promotes it surge
before ovulation.
AINA SAMSON is a Lecturer II at the Department of Physiology
AINA has a Ph.D in Reproduction/Endrocrinology from University of Lagos